Identification of genes that are inappropriately regulated in hereditary iron overload
Iron is an indispensable micronutrient for living organisms. Studies have shown that the Hfe gene plays a significant role in iron regulation in mammalian cells. However, it is unclear how gene expression is affected by the defective Hfe gene to elicit higher iron accumulation than normal iron levels, and if strain differential iron overload in Hfe knock-out (KO) mice is associated with strain specific interactions of the genes. This summer, I will be addressing these questions by using microarray: differentially expressed genes in Hfe KO mice will be determined in both AKR and C57BL/6 background rather than strain specific gene differences or secondary to high iron content. Genes that are specifically affected by Hfe gene disruption will also be determined.
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- Major: Molecular and Cell Biology
- Mentor: Chris Vulpe, Nutritional Sciences and Toxicology