Investigating the Role of Ras-like GTPases in TORC2 Localization of Saccharomyces cerevisiae
The Target of Rapamycin (TOR) is a protein kinase involved in many aspects of cell physiology. Because of its importance in regulating growth, TOR has been central to metabolic and cancer research for years. Saccharomyces cerevisiae contains two paralogs of TOR, Tor1 and Tor2, which exist in their respective multi-subunit complexes TORC1 and TORC2. TORC2 has been found to regulate cell polarity, endocytosis, and actin polymerization in response to external stimuli, and studies have shown that the localization of TORC2 to the plasma membrane is essential for the complex’s function. However, the upstream regulatory mechanisms of TORC2 still remain largely unknown. Ras proteins are of interest because they contain a C-terminal motif that targets the GTPase to the membrane, and relationships between homologs of TOR and Ras-GTPases have been found in other model organisms. Through this project, my main aim is to understand more about the potential relationship between TORC2 and Ras-GTPases of Saccharomyces cerevisiae by analyzing the role of Ras proteins in the complexs localization.
Message to Sponsor
- Major: Molecular & Cell Biology
- Mentor: Jeremy Thorner